Vol. 39, issue 04, article # 1

Sokolovskaya Yu. G., Krasnova E. D., Voronov D. A., Patsaeva S. V. Spectral and optical characteristics of dissolved organic matter in meromictic reservoirs of the White Sea coast. // Optika Atmosfery i Okeana. 2026. V. 39. No. 04. P. 281–288. DOI: 10.15372/AOO20260401 [in Russian].
Copy the reference to clipboard

Abstract:

Natural water contains dissolved organic matter (DOM), which plays an important role in biogeochemical processes and affects the functioning of aquatic ecosystems. The paper analyzes the spectral and luminescent characteristics (optical indices, difference absorption spectra, fluorescence quantum yield, and protein-like fluorescence) of DOM chromophoric fraction (CDOM) in two meromictic reservoirs on the coast of the Kandalaksha Bay of the White Sea, lakes Elovoe and Trekhtzvetnoe based on the results of expeditionary work in 2025. The difference in optical indices in different layers of the reservoirs (the surface fresh layer, the intermediate brackish aerobic layer, the chemocline, and saline bottom hydrogen sulfide zone) and their relationship with hydrochemical parameters are shown. An increase in the quantum yield of CDOM fluorescence in the chemocline of the Lake Elovoe and its decrease in the Lake Trekhtzvetnoe are explained. The results are of importance for ecological monitoring of aquatic ecosystems, as well as for understanding the processes that influence optical characteristics of natural CDOM.

Keywords:

dissolved organic matter (DOM), chromophoric fraction of DOM (CDOM), meromictic reservoir, White Sea, absorption spectra, excitation wavelength, fluorescence quantum yield

Figures:

References:

1. Stevenson F.J. Humus Chemistry. Genesis, Composition, Reactions. New York: John Wiley and Sons, 1994. P. 24–34.
2. Hedges J.I., Eglinton G., Hatcher P.G., Kirchman D.L., Arnosti C., Derenn S., Evershed R.P., de Leeuw J.W., Littke R., Michaelis W., Rullkötter J. The molecularly-uncharacterized component of nonliving organic matter in natural environments // Organic Geochem. 2000. V. 31. P. 945–958.
3. Stedmon C.A., Nelson N.B. The optical properties of DOM in the ocean // Biogeochemistry of Marine Dissolved Organic Matter / D.A. Hansell, C.A. Carlson (eds.). San Diego: Elsevier, 2015. P. 481–508.
4. Wünsch U., Murphy K., Stedmon C. Fluorescence quantum yields of natural organic matter and organic compounds: Implications for the fluorescence-based interpretation of organic matter composition // Front. Marine Sci. 2015. V. 2, Art. 98. P. 1–9. DOI: 10.3389/fmars.2015.00098.
5. Dittmar T., Lennartz S.T., Buck-Wiese H., Hansell D.A., Santinelli C., Vanni C., Blasius B., Hehemann J.-H. Enigmatic persistence of dissolved organic matter in the ocean // Nature Rev. Earth Environ. 2021. V. 2. P. 570–583. DOI: 10.1038/s43017-021-00183-7.
6. Romankevich E.A., Vetrov A.A., Peresypkin V.I. Organicheskoe veshchestvo Mirovogo okeana // Geologiya i geofizika. 2009. V. 50, N 4. P. 401–411.
7. Stedmon C.A., Amon R.M.W., Rinehart A.J., Walker S.A. The supply and characteristics of colored dissolved organic matter (CDOM) in the Arctic Ocean: Pan Arctic trends and differences // Marine Chem. 2011. V. 124, N 1–4. P. 108–118. DOI: 10.1016/j.marchem.2010.12.007.
8. Mann P., Spencer R., Hernes P., Six J., Aiken G., Tank S., McClelland J., Butler K., Dyda R., Holmes R. Pan-Arctic trends in terrestrial dissolved organic matter from optical measurements // Front. Earth Sci. V. 4. Art. 25. DOI: 10.3389/feart.2016.00025.
9. Gjelstrup C.V.B., Myers P.G., Lee C.M., Azetsu-Scott K., Stedmon C.A. Connectivity between Siberian river runoff and the lower limb of the Atlantic Meridi-onal Overturning Circulation // Limnol. Oceanogr. 2024. V. 69, N 11. P. 2680–2687. DOI: 10.1002/lno.12696.
10. Wünsch U.J., Gonçalves-Araujo R., Granskog M.A., Ebbesen L.G., Papadimitraki M., Stedmon C.A. Fluorescence as a tracer of the susceptibility of dissolved organic matter to photodegradation in the Arctic Ocean // Limnol. Oceanogr. 2025. V. 70, N 8. P. 2299–2314. DOI: 10.1002/lno.70119.
11. Ul'yantsev A.S., Ocherednik  V.V., Romankevich E.A. Primenenie dvumernogo fluorimetricheskogo skanirovaniya v analize organicheskogo veshchestva morskoi sredy // Dokl. RAN. 2015. V. 460, N 1. P. 93–97.
12. Glukhovets D.I., Gol'din Yu.A. Issledovanie svyazi solenosti i fluorestsentsii zheltogo veshchestva v Karskom more // Fund. i prikl. gidrofiz. 2018. V. 11, N 3. P. 34–40. DOI: 551.468.
13. Drozdova A.N., Patsaeva S.V., Khundzhua D.A. Fluorestsentsiya rastvorennogo organicheskogo veshchestva kak marker rasprostraneniya presnykh vod v Karskom more i zalivakh arkhipelaga Novaya Zemlya // Okeanologiya. 2019. V. 57, N 1. P. 49–56. DOI: 10.7868/S0030157417010038.
14. Zaitseva A.F., Konyukhov I.V., Kazimirko Y.V., Pogosyan S.I. Optical characteristics and distribution of chromophoric dissolved organic matter in Onega Bay (White Sea) during the summer season (findings from an expedition from June 22 to 26, 2015) // Oceanology. 2018. V. 58, N 2. P. 233–239. DOI: 10.1134/S0001437018020169.
15. Drozdova A.N. Opticheskie svoistva rastvorennogo organicheskogo veshchestva poverkhnostnogo sloya vody morya Laptevykh // Opt. i spektroskop. 2019. V. 126, N 3. P. 383–388.
16. Khundzhua D.A., Patsaeva S.V., Trubetskoi O.A., Trubetskaya O.E. Analiz rastvorennogo organicheskogo veshchestva presnovodnykh ozer Karelii obratno-fazovoi vysokoeffektivnoi zhidkostnoi khromatografiei s odnovremennoi registratsiei opticheskoi plotnosti i fluorestsentsii // Vestn. Mosk. un-ta. Ser. 3. 2017. N 1. P. 66–73. DOI: 10.3103/S0027134907060082.
17. Sokolovskaya Yu.G., Krasnova E.D., Voronov D.A., Matorin D.N., Zhiltsova A.A., Patsaeva S.V. Optical proxies of euxinia: Spectroscopic studies of CDOM, chlorophyll, and bacteriochlorophylls in the Lagoon on Zeleny Cape (the White Sea) // Photonics. 2023. V. 10, N 6. Art. 672. P. 1–18. DOI: 10.3390/photonics10060672.
18. Sokolovskaya Yu.G., Demidenko N.A., Krasnova E.D., Voronov D.A., Savichev A.S., Patsaeva S.V. Spektral'nye svoistva rastvorennogo organicheskogo veshchestva i ikh zavisimost' ot glubiny v iskusstvenno i estestvenno otdelennykh meromikticheskikh vodoemakh // Opt. i spektroskop. 2024. V. 132, N 4. P. 374–382.
19. Sokolovskaya Yu.G., Tsvetkova A.D., Krasnova E.D., Voronov D.A., Burikov S.A., Dolenko T.A., Patsaeva S.V. Spektry i kinetika fluorestsentsii rastvorennogo organicheskogo veshchestva v belomorskoi stratifitsirovannoi lagune v zimnii i letne-osennii sezony // Opt. i spektroskop. 2025. V. 133, N 5. P. 444–455. DOI: 10.61011/OS.2025.05.60781.25-25.
20. Krasnova E.D., Mardashova M.V. Kak morskoi zaliv prevrashchaetsya v ozero // Priroda. 2020. N 1. P. 16–27. DOI: 10.7868/S0032874X20010020.
21. Krasnova E.D. Ekologiya meromikticheskikh ozer Russia. 1. Pribrezhnye morskie vodoemy // Vodnye resursy. 2021. V. 48, N 3. P. 322–333. DOI: 10.31857/S0321059621030093.
22. Sokolovskaya Yu.G., Zhil'tsova A.A., Krasnova E.D., Voronov D.A., Patsaeva S.V. Spektral'no-lyuminestsentnye kharakteristiki rastvorennogo organicheskogo veshchestva v meromikticheskikh vodoemakh Kandalakshskogo zaliva Belogo morya // Opt. i spektroskop. 2023. V. 131, N 6. P. 872–879. DOI: 10.21883/OS.2023.06.55924.111-23.
23. Krasnova E., Matorin D., Belevich T., Efimova L., Kharcheva A., Kokryatskaya N., Losyuk G., Todorenko D., Voronov D., Patsaeva S. The characteristic pattern of multiple colored layers in coastal stratified lakes in the process of separation from the White Sea // J. Oceanolog. Limnol. 2018. V. 36. P. 1962–1977. DOI: 10.1007/s00343-018-7323-2.
24. Savvichev A.S., Babenko V.V., Lunina O.N., Letarova M.A., Boldyreva D.I., Veslopolova E.F., Demidenko N.A., Kokryatskaya N.M., Krasnova E.D., Gaisin V.A., Kostryukova E.S., Gorlenko V.M., Letarov A.V. Sharp water column stratification with an extremely dense microbial population in a small meromictic lake Trekhtzvetnoe // Environ. Microbiol. 2018. V. 20, N 10. P. 3784. DOI: 10.1111/1462-2920.14384.
25. Krasnova E.D., Kharcheva A.V., Milyutina I.A., Voronov D.A., Patsaeva S.V. Study of microbial communities in redox zone of meromictic lakes isolated from the White Sea using spectral and molecular methods // J. Mar. Biol. Assoc. UK. 2015. V. 95, N 8. P. 1579–1590. DOI: 10.1017/S0025315415000582.
26. Kharcheva A.V., Zhil'tsova A.A., Lunina O.N., Krasnova E.D., Voronov D.A., Savichev A.S., Patsaeva S.V. Fluorestsentsiya bakteriokhlorofillov zelenykh sernykh bakterii v anaerobnoi zone dvukh prirodnykh vodoemov // Vestn. Mosk. un-ta. Ser. 3: Fiz., astr. 2018. N 4. P. 40–45.
27. Lakowicz J.R. Principles of Fluorescence Spectroscopy. New York: Springer, 2006. DOI: 10.1007/78-0-387-46312-4.
28. Eaton D.F. Reference materials for fluorescence measurement // Pure Appl. Chem. 1988. V. 60, N 7. P. 1107–1114.
29. Lunina O.N., Gruzdev D.S., Patsaeva S.V., Zhil'tsova A.A., Suzina N.E., Krasnova E.D., Voronov D.A., Kokryatskaya N.M., Veslopolova E.F., Savichev A.S. Anoksigennye fototrofnye bakterii meromikticheskogo ozera Bol'shie Khruslomeny (o. Olenii, Kandalakshskii zaliv, Murmanskaya oblast', Rossiya) // Mikrobiologiya. 2023. V. 92, N 6. P. 564–580. DOI: 10.31857/S0026365623600268.
30. Emel'yantsev P.S., Zhil'tsova A.A., Krasnova E.D., Voronov D.A., Rymar' V.V., Patsaeva S.V. Opredelenie kontsentratsii khlorosomnykh bakteriokhlorofillov po spektram pogloshcheniya kletok zelenykh sernykh bakterii v probakh prirodnoi vody // Vestn. Mosk. un-ta. Ser. 3. 2020. N 2. P. 25–30.
31. Zhiltsova A.A., Filippova O.A., Krasnova E.D., Voronov D.A., Patsaeva S.V. Sravnitel'nyi analiz spektral'nykh metodov opredeleniya kontsentratsii bakteriokhlorofilla d zelenykh sernykh bakterii v vode // Optika atmosf. i okeana. 2022. V. 35, N 4. P. 312–318. DOI: 10.15372/AOO20220411; Zhiltsova A.A., Filippova O.A., Krasnova E.D., Voronov D.A., Patsaeva S.V. Comparative analysis of spectral methods for determining bacteriochlorophyll d concentration in green sulfur bacteria in water // Atmos. Ocean. Opt. 2022. V. 35, N 5. P. 5627–568.
32. Losyuk G.N., Kokryatskaya N.M., Krasnova E.D. Serovodorodnoe zarazhenie pribrezhnykh ozer na raznykh stadiyakh izolyatsii ot Belogo morya // Okeanologiya. 2021. V. 61, N 3. P. 401–412. DOI: 10.31857/S003015742102012X.
33. Drozdova A.N., Kravchishina M.D., Khundzhua D.A., Freidkin M.P., Patsaeva S.V. Fluorescence quantum yield of CDOM in coastal zones of the Arctic seas // Int. J. Rem. Sens. 2018. V. 39, N 24. P. 9356–9379. DOI: 10.1080/01431161.2018.1506187.
34. Stedmon C.A., Thomas D.N., Papadimitriou S., Granskog M.A., Dieckmann G.S. Using fluorescence to characterize dissolved organic matter in Antarctic sea ice brines // J. Geophys. Res. Biogeosci. 2011. V. 116, N G03027. P. 1–9. DOI: 10.1029/2011jg001716.
35. Trubetskaya O., Richard C., Trubetskoj O. High amounts of free aromatic amino acids in the protein-like fluorescence of water-dissolved organic matter // Environ. Chem. Lett. 2016. V. 14, N 4. P. 495–500. DOI: 10.1007/s10311-016-0556-4.
36. Trubetskoi O.A., Trubetskaya O.E. Analiz fluoroforov rastvorennogo organicheskogo veshchestva vod reki Suvani metodom obratno-fazovoi zhidkostnoi khromatografii // Vodnye resursy. 2019. V. 46, N 4. P. 428–437. DOI: 10.31857/S0321-0596464428-437.
37. Sierra M.М.D., Donard O.F.X., Lamotte M., Belin С., Ewald М. Fluorescence spectroscopy of coastal and marine waters // Mar. Chem. 1994. V. 47. P. 127–144.
38. Parlanti E., Worz K., Geoffroy L., Lamotte M. Dissolved organic matter fluorescence spectroscopy as a tool to estimate biological activity in a coastal zone submitted to anthropogenic inputs // Org. Geochem. 2000. V. 31, N 12. P. 1765–1781. DOI: 10.1016/S0146-6380(00)00124-8.
39. Green S.A., Blough N.V. Optical absorption and fluorescence properties of chromophoric dissolved organic-matter in natural-waters // Limnol. Oceanogr. 1994. V. 39. P. 1903–1916. DOI: 10.4319/lo.1994.39.8.1903.
40. Andrew A.A., Del Vecchio R., Subramaniam A., Blough N.V. Chromophoric dissolved organic matter (CDOM) in the Equatorial Atlantic Ocean: Optical properties and their relation to CDOM structure and source // Mar. Chem. 2013. V. 148. P. 33–43. DOI: 10.1016/j.marchem.2012.11.001.