Optika Atmosfery i Okeana Journal

Vol. 19, issue 02-03, article # 19

Avrorov P. A., Klimkin A. V., Maltsev V. P., Semyanov K. A., Tarasov P. A. Determination of size parameters of isolated microparticles from data on the scattering phase function. // Atmospheric and oceanic optics. 2006. V. 19. No. 02-03. P. 179-182. PDF
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Abstract:

Scanning flow cytometer allows one to measure continuously the angular dependence of light scattering by isolated microparticles in the range of scattering angles from 10 to 70 degrees. At present, cytometers are mostly used in characterization of biological cells and bacteria in water. Their typical sizes are from fractions to tens micrometers, with the refractive indices being from 1.36 to 1.7. A simple method to determine parameters of a microparticle, based on analysis of Fourier spectrum of a modified scattering phase function, is considered. The peaks observed in Fourier spectrum are related to resonances on characteristic dimensions of particles. For the case of homogeneous spheres, this method allows one to precisely determine the particle diameter. We suggest using similar approach to characterize particles of arbitrary shapes. Results for blood cells of platelets, erythrocytes, and leukocytes are presented.