Vol. 31, issue 02, article # 4

Paramonov L.E. Spectrum of absorption coefficients and intracellular concentration of cyanobacterial pigments by the example of Spirulina platensis. // Optika Atmosfery i Okeana. 2018. V. 31. No. 02. P. 103–108 [in Russian].
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An analytical estimate of the absorption cross section for homogeneous nonspherical particles and their suspensions is suggested and justified. On the example of Spirulina platensis, a method for evaluating the spectrum of pigment absorption for photosynthetically active radiation, excluding the use of pigment extracts, is considered. The effect of the size and shape distribution of cells on the absorption spectra is studied. The intracellular concentrations of chlorophyll a, phycoerythrin, and phycocyanin are estimated.


spectrum of absorption coefficients, Spirulina platensis, intracellular concentration of pigments, chlorophyll a, phycocyanin, phycoerythrin


  1. Morel A. Light and marine photosynthesis: A spectral model with geochemical and climatological implications // Prog. Oceanogr. 1991. V. 26. P. 263–306.
  2. Platt T., Sathyendranath S. Oceanic primary production: Estimation by remote sensing at local and regional scales // Science. 1988. V. 241. P. 1613–1620.
  3. Bricaud A., Morel A., Babin M., Allali K., Claustre H. Variations of light absorption by suspended particles with chlorophyll a concentration in oceanic (case 1) waters: Analysis and implications for bio-optical models // J. Geophys. Res. 1998. V. 103. P. 31033–31044.
  4. Sathyendranath S., Cota G., Stuart V., Maass H., Platt T. Remote sensing of phytoplankton pigments: A comparison of empirical and theoretical approaches // Int. J. Remote Sens. 2001. V. 22. P. 249–273.
  5. Ficek D., Kaczmarek S., Ston-Egiert J., Wozniak B., Majchrowski R., Dera J. Spectra of light absorption by phytoplankton pigments in the Baltic: Conclusions to be drawn from a Gaussian analysis of empirical data // Oceanologia. 2004. V. 46. P. 533–555.
  6. Kirk J.T.O. Light and photosynthesis in aquatic ecosystems. 3rd ed. Cambridge: University Press, 2011. 649 p.
  7. Duysens L.M.N. The flattening effect of the absorption spectra of suspensions as compared to that of solutions // Biochim. Biophys. Acta. 1956. V. 19. P. 1–12.
  8. Hoepffner N., Sathyendranath S. Effect of pigment composition on absorption properties of phytoplankton // Mar. Ecol.: Prog. Ser. 1991. V. 73. P. 11–23.
  9. Simis S.G.H., Kauko H.M. In vivo mass-specific absorption spectra of phycobilipigments through selective bleaching // Limnol. Oceanogr.: Methods. 2012. V. 10. P. 214–226.
  10. Paramonov L.E., Hromechek E.B., Abdulkin V.V., Shmidt V.A. K resheniju obratnyh zadach na klassah jekvivalentnosti // Optika atmosf. i okeana. 2004. V. 17, N 5–6. P. 508–512.
  11. Van de Hjulst G. Rassejanie sveta malymi chasticami / per. s angl. M.: Izd-vo inostr. lit-ry, 1961. 536 p.
  12. Shifrin K.S., Tonna G. Prostaja formula dlja kojefficienta pogloshhenija slaboprelomljajushhih chastic // Optika i spektroskopija. 1992. V. 72. P. 487–490.
  13. Paramonov L.E. Opticheskaja jekvivalentnost' izotropnyh ansamblej jellipsoidal'nyh chastic v priblizhenijah Rjeleja–Gansa–Debaja i anomal'noj difrakcii, i ee sledstvija // Optika i spektroskopija. 2012. V. 112, N 5. P. 853–861.
  14. Waterman P.C. Symmetry, unitarity and geometry in electromagnetic scattering // Phys. Rev. D. 1970. V. 3. P. 825–839.
  15. Paramonov L.E. T-matrix approach and the angular momentum theory in light-scattering problems by ensembles of arbitrarily shaped particles // J. Opt. Soc. Am. A. 1995. V. 12. P. 2698–2707.
  16. Kirk J.T.O. A theoretical analysis of the contribution of algal cells to the attenuation of light within natural waters. III. Cylindrical and spheroidal cells // New Phytol. 1976. V. 77, N 2. P. 341 –358.
  17. Kuz'minov F.I., Shirshin E.A., Gorbunov M.Ju., Fadeev V.V. Novye opticheskie podhody v issledovanii fotofiziologicheskih harakteristik cianobakterij in situ // Fundam. i prikl. gidrofiz. 2015. V. 8. P. 41–47.