Vol. 21, issue 05, article # 2

Lavric N. L., Efimov Yu. Ya., Mulloev N. U. Inner-filter effect as a function of the diameter of an exciting beam in fluorescence emitting spectra. // Atmospheric and oceanic optics. 2008. V. 21. No. 05. P. 331-334. PDF
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Abstract:

A formula is obtained allowing calculation of the fluorescence intensity in an absorbing medium as a function of the laser beam diameter, the distance between the laser beam center and the cell exit wall, and optical density per unit of the length. The formula was applied to calculations of corrections, which make it possible to take into account the effect of the secondary inner-filter (IFE) in particular experiments. These results are compared with revealing of differences, with the results of IFE calculations performed under the assumption of an infinitely narrow exciting beam, when the IFE can be considered strictly through the use of the Bouguer-Lambert-Beer law. A possibility of determining optical densities in a spectrometer, detecting fluorescence spectra, is demonstrated by the method of variation of the distance between the laser beam and the wall of the working cell.