Fluorescence of centrifuged seawater samples taken from a depth of 20 to 200 m in the Black Sea was investigated. Intense ultraviolet fluorescence of dissolved organic seawater matter with two peaks near 345 and 358 nm was found. The fluorescence is excited by a band with two peaks at 260 and 290 nm. With depth, the intensity of the fluorescence decreases, and the shape of the fluorescence spectrum changes. In the sea photic zone, the character of ultraviolet fluorescence of the dissolved organic matter changes in different seasons.