In this paper we consider the influence of the total ozone variations on the change of the level of UV-В solar radiation at the ground in midlatitudes of the Northern hemisphere. We have analyzed the spectrum of radiation. It has been established that correlation between relative deviations of the diurnal doses of UV-В radiation and of the total ozone from the standard ones is statistically significant even for the confidence probability of 0.99. We have investigated the influence of smoothing by a sliding mean as well as of averaging over 10 days, a month, and a year on the level of correlation. It is shown that in studying the influence of solar UV-В radiation with the wavelengths shorter than 315 nm on the biosphere of the middle- and high-latitude regions with high total ozone content, the series of data on TOC can adequately substitute the missing data of long-term series radiation measurements.